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nfectious Diseases

Principal Investigators

Huanyu Dou, M.D.

Assistant Professor, Department of Biomedical Sciences and Center of Excellence in Infectious Diseases


Department of Biomedical Sciences
Center of Excellence in Infectious Diseases
Texas Tech University Health Sciences Center
5001 El Paso Drive, El Paso TX, Zip Code 79905
Phone: (915) 215-4259
Huanyu.Dou@ttuhsc.edu


Research Interests

The laboratory's primary objective is to investigate the therapeutic role of a cell-based nanodrug delivery system with characterization of nanocarrier, bone marrow derived cells (BMDC) migration into the brain. In vivo and ex in vivo studies are being conducted to elucidate coupled immunoglobulin to nanoformulated drug specific uptake by BMDC in order to target areas of inflammation in brain disease area.

Long-range Goals

To study the therapeutic role of a cell-based nanodrug delivery system

Techniques used in the laboratory

  • Stereotactic brain injection
  • Nanoformulation
  • Histology
  • Western Blot, RT-PCR, HPLC
  • Cell and tissue cultures
  • AFM
  • Live confocal microscopy
  • Stem cell isolation

Research Goals

A cell-based targeting delivery system in mouse model of HIV-1 infection and neuroAIDS is being developed. Neuroprotective and antiviral targeting delivery system will have been developed using BMDC carrying nanoformulations to target the diseased areas. Track BMDC migration and HPLC assay for the elucidation of BMDC anatomical distribution and therapeutics pharmacokinetics.

Animal models of tumor growth (i.e. primary and cell line) and experimental therapy are being used to test several antitumor drugs including BMDC nanoparticles delivery of chemotheraputics and anti-tumor molecules. The combination of paclitaxel and temozolomide is being investigated to establish a BMDC nanoparticle(s) delivery system in a mouse model of human glioma. This is a novel therapeutic approach for chemotherapy.

Nanotechnology and synthesis have been used to couple immunoglobulin to nanoparticles in order to facilitate uptake and delivery molecule therapeutics into the d the brain. The studies will focus on how nanoformulation and/or antibody binded nanoparticles can facilitate delivery to cross the BBB. The development of nanoformatuion of anti-inflammation and neuroprotection are being done in collaborations with Drs. Jun Chen, John Zhang and Christopher J. Destache. Cellular receptor-mediated nano-drug delivery would improve brain drug levels through the blood-brain barrier (BBB), eliciting improvement of therapeutic efficacy.

Positions and Employment

2008-2010 Assistant Professor, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE
2005-2008 Director, Neurotherapeutics Program and Instructor (faculty), Department of Pharmacology and Experimental Neuroscience, Center for Neurovirology and Neurodegenerative Disorders, UNMC, Omaha, NE
2002-2004 Post-doctoral research associate, Center for Neurovirology and Neurodegenerative Disorders, Department of Pathology and Microbiology, UNMC, Omaha, NE
2000-2001 Post-doctoral fellow, Laboratory of Zvi Malik, Unit of Microscopy, Faculty of Life, Sciences of Bar-Ilan University, Israel
1995-2000 Associate Professor and Vice-Director, Ceter Morphology, Department of Anatomy, Weifang Medical College, China
1993-1995 Assistant Professor, Department of Anatomy, Weifang Medical College, China
1985-1990 Doctor and Instructor, Department of Anatomy, Weifang Medical College, China

Other experience and professional membership

  • Society for Neuroscience
  • International Society of Neuroimmunology
  • International Society for Neurochemistry
  • Society on NeuroImmune Pharmacology
  • International Association of Nanotechnology
  • Society for Translational Neuroscience

Recent Publications

  1. Dou H, GrotepasCB, ZengC, BoskaMD, DestacheCJ,ChaubalM, WerlingJ, KippJ,RabinowBE,GendelmanHE. (2009). Macrophage delivery of nanoformulated anti retroviral drug to the brain in a murine model of neuroAIDS.  J. of Immunology, 183(1):661-669.
  2. Beduneau A, Ma Z, Grotepas CB, Kabanov A, Rabinow BE, Gong N, Mosley RL, Dou H, Boska MD, Gendelman HE. (2009). Facilitated monocyte-macrophage uptake and tissue distribution of superparmagnetic iron-oxide nanoparticles. PLoS One.4(2):e4343.
  3. Keblesh JP, Dou H, Gendelman HE, Xiong H. (2009). 4-Aminopyridine improves spatial memory in a murine model of HIV-1 encephalitis. J Neuroimmune Pharmacol. 4(3):317-327.  
  4. Eggert D, Dash PK, Serradji N, Dong CZ, Clayette P, Heymans F, Dou H, Gorantla S, Gelbard HA, Poluektova L, Gendelman HE. (2009). Development of a platelet-activating factor antagonist for HIV-1 associated neurocognitive disorders. J Neuroimmunol. 213(1-2):47-59.
  5. Nowacek AS, Miller RL, McMillan J, Kanmogne G, Kanmogne M, Mosley RL, Ma Z, Graham S, Chaubal M, Werling J, Rabinow B, Dou H, Gendelman HE. (2009). NanoART synthesis, characterization, uptake, release and toxicology for human monocyte-macrophage drug delivery.Nanomedicine (Lond). 4(8):903-917.
  6. Quan LD, Purdue PE, Liu XM, Boska MD, Lele SM, Thiele GM, Mikuls TR, Dou H, Goldring SR, Wang . (2010). Development of a macromolecular prodrug for the treatment of inflammatory arthritis: mechanisms involved in arthrotropism and sustained therapeutic efficacy. Arthritis Res Ther.;12(5):R170.
  7. Eggert D, Dash PK, Gorantla S, Dou H, Schifitto G, Maggirwar SB, Dewhurst S, Poluektova L, Gelbard HA, Gendelman HE.  (2010). Neuroprotective activities of CEP-1347 in models of neuroAIDS. J Immunol. 184(2):746-56.
  8. Chen H, BurrisM, Spagnoli F, RollandWB, DouH; Tang J, Zhang JH. (2012). Nano-erythropoietin is ten times more effective than regular Erythropoietin in neuroprotection in a neonatal rat model of hypoxia and ischemia. Stroke 43: 884-887.
  9. Tao Yo, Han J, Ye, C, Thomas and Dou H. (2012). Reduction-responsive gold-nanoparticle-conjugated Pluronic micelles: an effective anti-cancer drug delivery system. J. Mater. Chem. 22: 18864-18871.
  10. Wu Y, Chen Q, Peng H, Dou H, Zhou Y, Huang Y, Zheng JC (2012). Directed migration of human neural progenitor cells to interleukin-1β is promoted by chemokines stromal cell-derived factor-1 and monocyte chemotactic factor-1 in mouse brains. Transl Neurodegener. 1(1):15.
  11. Tao, Y, Han H, and DouH (2012). Brain-targeting gene delivery using a rabies virus glycoprotein peptidemodulated hollow liposome: bio-behavioral study. J. Mater. Chem., 22: 11808-11815.
  12. Tao, Y, Han H, and DouH. (2012).  Surface modification of paclitaxel-loaded polymeric nanoparticles: Evaluation of in vitro cellular behavior and in vivo pharmacokinetic. Polymer 53:  5078-5086.
  13. Tao Yo, Han J and Dou H. (2012). Paclitaxel-loaded tocopheryl succinate-conjugated chitosan oligosaccharide nanoparticles for synergistic chemotherapy. J. Mater. Chem.,22: 8930-9837.
  14. Tao, Y, NingM, and DouH. (2013). A Novel Therapeutic System for Malignant Glioma:  Nanoformulation, Pharmacokinetic and Anti-Cancer Properties of Cell-Nano-Drug delivery. Nanomedicine. 9 (2): 222-232.
  15. Tao Y, Han J, Wang X, Dou H. (2013). Nano-formulation of paclitaxel by vitamin E succinate functionalized pluronic micelles for enhanced encapsulation, stability and cytotoxicity. Colloids Surf B Biointerfaces. 102: 604-610.

Staff

Lei Zou, Ph.D

Post Doctoral Research Associate

(915)215-4260

Tima Thomas, M.S.

Lead Technician

(915)215-4275

Juan Rodriguez, M.D., Ph.D.

Visiting Scientist

 


Collaborators

Jun Chen, M.D.

Professor, Department of Neurology

University of Pittsburgh medical Center

Christopher Destache, Pharm.D.

Professor, Department of Pharmacy Practice

Creighton University 

 Yan Xu, Ph.D

 Professor of Anesthesiology, Pharmacology & Chemical Biology and Structural Biology

Vice Chairman for Basic Sciences of Department of Anesthesiology

University of Pittsburgh School of Medicine

John Zhang, MD, PhD.

Professor, Departments of Neurosurgery, Anesthesiology, Physiology and Pharmacology, Pathology and Human Anatomy

Loma Linda University School of Medicine

 

 

 

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